Equipment

ÄKTAxpress

ÄKTAxpress for protein purification gives you the highest possible purity needed for structural and functional studies. Optimized protocols with a choice of up to four purification steps minimizes the need for chromatography expertise. Tag removal and maintenance procedures can be integrated into the purification protocols, eliminating manual interference during a run. Purification schemes for double affinity-tagged proteins are also supported. A four-step protocol may consist of affinity chromatography (AC), desalting (DS), ion exchange chromatography (IEX) and gel filtration (GF).

ÄKTAxpress™ is a dedicated chromatography system for multistep purification of tagged proteins and antibodies. Each ÄKTAxpress module consists of a system and sample pump (0.5 - 65 ml/min), an air sensor for complete sample application, a UV monitor (280 or 254 nm), and a 96- or 24-well microplate fraction collector. All components are under the control of a specially designed version of UNICORN control software. The system also accommodates bar-code readers for further process automation.

ÄKTA Purifier

ÄKTA™purifier systems are designed for fast, high-resolution separation and characterization of proteins at laboratory scale. The systems perform all chromatographic techniques and scout for optimal binding and elution conditions, pH, gradient shapes, and flow rates. This system can produce 25 MPa and flow rates up to 10 ml/min, become ideal for laboratory purification and high-resolution analysis.

ÄKTA Prime

ÄKTAprime™ prime is a compact one-step purification system that operates with most prepacked columns.  The system has preprogrammed methods for typical applications such as purification of tagged proteins, Mabs and sample clean-up. The flexible system can also be used for more general purification tasks such as gel filtration, ion exchange, HIC, and affinity chromatography.

The unit is compact and comprises a system pump, fraction collector, monitors for UV, and conductivity. Valves for buffer selection, sample injection, gradient formation, and flow diversion are also integrated into the system. The system is not PC controlled but together with PrimeView™ software it offers continuous real-time monitoring and evaluation of results.

Bioreactor (Eucaryote) BelloCell 3000

The eucaryote cell culture system BelloCell 3000  provides a protected, controlled, and contained environment for the growth of cell cultures. Maximal capacity is equivalent to extremely high yields — averaging 2.4 x 1010cells for a system with four bottles. The BelloCell 3000 consists of three major components; a control box, the BelloStage unit, and ready-to-use disposable 500 ml bottles. The BelloStage unit, which holds up to four disposable cell culture bottles, moves the bottles’ contents up and down according to your program, using a platform to compress and expand the bellows built into each bottle, to optimize oxygenation. As the platform lifts, it compresses the bellows, sending the media into the chamber that contains the BioNOC II® disks; as the platform descends, the media returns to the expanding bellows, exposing the carrier disks to the atmospheric environment. The growth cells in these carriers grow along the fibers, and then pile up to fill the space in the net.

Multitron 2

The Multitron II is a large capacity shaking incubator which combines flexibility and operational safety with the optimum utilization of space by its modular construction. UPR laboratory have three units with culture capacity of 5 liters each one for differents procariotyc cultures. In addition integral microprocessor offers a wide variety of control possibilities, including high temperature range (Cº 12 to Cº 65), oxygenation control and light intensity.